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Skyline Online 2025

The Skyline Team and our invited instructors are pleased to announce the return of the wildly popular Skyline Online course coming this October 2025. These sessions have always filled up quickly. Do not put off registering if you want to attend.

Skyline Online will offer two 2-day sessions on Proteomics (3, 4) topics and a 3-day session on Small Molecule (1-and-2) topics:

-Session 1 and 2 - Intro and Advanced Small Molecule Topics - BONUS! 3 Days, same price - Oct 8-10 -Session 3 - Introduction to Skyline for Proteomics - Oct 14-15 -Session 4 - Advanced Proteomics Topics - Oct 17-18

Register Now

course skyline webinar


Weeklong Skyline Course in Seattle

Weeklong Skyline Course in Seattle! Great speakers and participation in our yearly course.

courses skyline


Transformer Model for DIA Data Published in Nature Methods

New Publication in Nature Methods. A transformer model for de novo sequencing of data-independent acquisition mass spectrometry data. Great collaborative project pushed by Justin Sanders in the Noble Lab. If you asked me 5 years ago if it would be possible to use a de novo tool on DIA data, I would have thought it would only exist in science fiction. Love being proven wrong.

publications nature-methods dia ai


Mag-Net Paper Published in Nature Communications

The Mag-Net paper is out in Nature Communications. Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome. This started as a pandemic side project of Chris Wu’s and we now have many variants on the idea. We’ve now put 1000s of plasma samples through this protocol and we know others have too. This has been a game changer for how we view biofluid proteomics.

publications nature-communications plasma mag-net EV


Skyline User Group Meeting at ASMS 2025

Skyline User Group Meeting at ASMS. Another year and another fantastic user group meeting. We had 10 fantastic talks from colleagues describing how they are using Skyline in their research. See recordings on the Skyline website.

conferences skyline asms